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. 2019 Jul 15;8(14):e012486. doi: 10.1161/JAHA.119.012486

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© 2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Picrosirius red staining for collagen. A, Representative images of picrosirius red–stained sections through the left ventricle (LV) of the heart. B, Representative high‐resolution images corresponding to the insets in (A). C, Morphometric quantification of collagen (percentage picrosirius red–positive area relative to the total LV area), **P<0.01, ****P<0.0001. eTNAP indicates overexpression of tissue‐nonspecific alkaline phosphatase in the endothelium; hArg, homoarginine; WHC, wicked high‐cholesterol allele.