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. 2019 Jul 23;14:5697–5711. doi: 10.2147/IJN.S214363

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© 2019 Park et al.

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(A) Quantitative real-time PCR analysis of the mRNA levels of the transcription factor genes for osteogenic differentiation (Dlx5, Runx2 and osterix) and osteoblast phenotype genes (type I collagen [COL], ALP, bone sialoprotein [BSP] and osteocalcin [OC]) at 7 days of culture. *P<0.05 compared with the other surface; ^#P<0.05 between two surfaces. (B) ELISA result for the detection of type I COL protein levels secreted into the cell culture media by MSCs grown on the investigated surfaces after 7 days of incubation. *P<0.05 compared with the other surface. (C) ALP activity of MSCs at 7 days of culture. *P<0.05 compared with the other surface; ^#P<0.05 between the two surfaces. The values are the mean ± SD of three independent experiments.