Alternol enhances anti-oxidant enzymatic activities in BPH1 cells. A-B; Cells were seeded in 6-well plates and treated with DMSO or Alternol (10 μM) for 4 h. Cellular proteins were extracted for SDO or catalase activity with the colorimetric assay kits from BioVision.
C; Cells were left untreated or treated with Alternol (10 μM) for 2–4 h and cellular contents of GSH and GSSG were assessed with the assay kit from Enzo Life as described in the text.
Data are presented as the MEAN from three separated experiments and the error bar indicates the SEM of the MEAN. The statistical significance (Student t-test, p <0.05) was indicated with the asterisk compared to the DMSO control or the hashtag compared to BPH1 cells.