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. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Kidney Int. 2018 Nov;94(5):1002–1012. doi: 10.1016/j.kint.2018.08.011

Figure 12: FGF23 decreases osteocyte apoptosis in vitro.

Figure 12:

A) Ethidium bromide (Et.Br)/acridine orange (Ac.O) staining of serum deprived MLO-Y4 cells after 48 hours in 100 nM FGF23. B) The percentage of dead cells, as defined by the percentage of cells staining with Et.Br (orange) relative to the total number of live cells staining with Ac.O (green), after 48 hours in culture under control (3mM phosphate) and high phosphate (10mM phosphate) conditions with varying (0 and 100 ng/mL) FGF23 concentrations. Values represent the average ± standard error for n=8 replicates. The asterisk indicates a difference (p<0.01) from cells treated with 10 mM Pi.