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. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Kidney Int. 2018 Nov;94(5):1002–1012. doi: 10.1016/j.kint.2018.08.011

Figure 8: Mineralization of primary CKD osteoblasts is impaired in vitro.

Figure 8:

(A) Alizarin red S staining of primary osteoblasts from 2 separate representative healthy controls and 2 separate representative dialysis patients grown to confluence and then cultured under standard pro-mineralizing conditions for 2 weeks. (B) Quantification of extracted Alizarin Red S dye from healthy control and pediatric dialysis patient primary osteoblasts grown to confluence and cultured under standard pro-mineralizing conditions for 3 weeks. Values represent the mean ± standard error values for the 3 controls and 6 patients. The asterisk indicates a between-group difference in mineral accumulation at 2 weeks in culture.