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. 2019 Apr 17;6(12):1802136. doi: 10.1002/advs.201802136

Figure 3.

Figure 3

ESCs culturing on RGO substrates possess multilineage differentiation potential. a) The relative levels of pluripotency gene (Esrrb, Klf4, and Rex1) and mesendoderm gene (Fgf5, Otx2, Eomes, Dnmt3b, and Gata6) during early differentiation expressed by 46C cultured on feeder, matrigel, and RGO‐30 substrates. b) The relative expression levels of Esrrb, Klf4, Rex1, Nestin, N‐cad (N‐cadherin), Zfp521 (zinc finger protein 521), and Tuj1 in 46C cultured on feeder, matrigel, and RGO‐30 substrates were assessed by qRT‐PCR. Nestin, N‐cad, and Zfp521 are markers of ectoderm, and Tuj1 is a marker of neuron. # p < 0.05 versus matrigel group.