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Long‐term cultivation of ESC on RGO substrate. a) qRT‐PCR was used to detect the expression level of pluripotency gene after continuous subculture of 46C on feeder, matrigel, and RGO‐30 substrates. # p < 0.05, ## p < 0.01 versus matrigel group, and * p < 0.05, ** p < 0.01 versus feeder group. b) The expression levels of Nanog(green) and SSEA1(red) in 46C after continuous subculture was tested by immunofluorescent staining. P3/6/9/12/15 stand for passage 3/6/9/12/15. Scale bar: 50 µm.
