Table 1.
Internal transcribed spacer (ITS) ribotypes, plastid haplotypes and bottleneck analysis of R. prainii and R. stapfii populations
| Pop ID | Individual numbers | ITS ribotype (numbers) | Plastid haplotype (numbers) | SSR | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| H e | H o | N e | P signtest | P stdv | P wilcoxon | Freq (private allele) | ||||
| Pra1 | 12 | Hap1 (24) | Chap1 (12) | 0.467 | 0.392 | 23.38 | 0.076 | 0.040* | 0.322 | 0 |
| Pra2 | 12 |
Hap1 (14) Hap2 (1) Hap3 (3) Hap4 (3) |
Chap2 (12) | 0.411 | 0.315 | 22.77 | 0.366 | 0.152 | 0.414 | 0.046 |
| Pra3 | 12 |
Hap1 (17) Hap6 (2) Hap8 (2) Hap12 (3) |
Chap4 (11) Chap5 (1) |
0.408 | 0.303 | 21.69 | 0.304 | 0.120 | 0.557 | 0.002 |
| Pra4 | 11 |
Hap1 (15) Hap6 (4) Hap8 (2) Hap12 (1) |
Chap4 (11) | 0.446 | 0.332 | 20.15 | 0.264 | 0.359 | 0.625 | 0.004 |
| Pra5 | 12 |
Hap1 (16) Hap6 (8) |
Chap6 (12) | 0.457 | 0.404 | 23.54 | 0.247 | 0.104 | 0.432 | 0.003 |
| Pra6 | 11 | Hap1 (22) |
Chap4 (9) Chap5 (2) |
0.468 | 0.343 | 21.85 | 0.399 | 0.273 | 0.359 | 0 |
| Pra7 | 13 |
Hap1 (17) Hap5 (2) Hap6 (4) Hap7 (3) |
Chap5 (12) Chap6 (1) |
0.423 | 0.314 | 24.77 | 0.182 | 0.095 | 0.206 | 0.006 |
| Pra8 | 14 |
Hap1 (25) Hap8 (3) |
Chap4 (14) | 0.314 | 0.317 | 27.69 | 0.585 | 0.009** | 0.570 | 0.011 |
| Pra9 | 13 |
Hap1 (24) Hap8 (2) |
Chap4 (13) | 0.431 | 0.343 | 25.69 | 0.071 | 0.018* | 0.193 | 0.004 |
| Pra10 | 12 | Hap1 (24) |
Chap4 (11) Chap5 (1) |
0.355 | 0.292 | 23.85 | 0.549 | 0.025* | 0.232 | 0.012 |
| Pra1−10 | 122 | Hap1‐Hap8, Hap12 | Chap1, 2, 4, 5 | 0.409 | 0.264 | 235.38 | 0.000** | 0.000** | 0.000** | ‐ |
| Pra11 | 12 |
Hap9 (20) Hap10 (1) Hap11 (3) |
Chap3 (12) | 0.503 | 0.427 | 23.69 | 0.565 | 0.227 | 0.547 | 0.215 |
| Sta1 | 15 | Hap13 (30) | Chap7 (15) | 0.355 | 0.340 | 29.69 | 0.049* | 0.001** | 0.020* | 0.005 |
| Sta2 | 14 | Hap13 (28) | Chap7 (14) | 0.335 | 0.338 | 27.69 | 0.422 | 0.440 | 0.938 | 0 |
| Sta3 | 11 | Hap13 (22) | Chap7 (11) | 0.397 | 0.387 | 21.54 | 0.576 | 0.419 | 0.844 | 0 |
| Sta4 | 13 | Hap13 (26) |
Chap7 (11) Chap8 (1) Chap9 (1) |
0.36 | 0.39 | 25.38 | 0.461 | 0.432 | 1.000 | 0 |
| Sta5 | 13 | Hap13 (26) |
Chap7 (12) Chap8 (1) |
0.407 | 0.349 | 25.85 | 0.229 | 0.127 | 0.688 | 0 |
| Sta6 | 12 | Hap13 (24) | Chap7 (12) | 0.291 | 0.263 | 23.69 | 0.203 | 0.039* | 0.109 | 0 |
| Sta7 | 17 | Hap13 (34) |
Chap7 (15) Chap8 (1) Chap9 (1) |
0.348 | 0.338 | 33.08 | 0.446 | 0.224 | 0.469 | 0 |
| Sta8 | 11 | Hap14 (22) | Chap7 (11) | 0.303 | 0.251 | 21.23 | 0.061 | 0.009** | 0.031* | 0.006 |
| Sta1‐8 | 106 | Hap13, 14 | Chap7, 8, 9 | 0.258 | 0.223 | 208.15 | 0.001** | 0.000** | 0.001** | ‐ |
| Total | 240 | Hap1‐14 | Chap1‐9 | 0.576 | 0.223 | 467.23 | 0.001** | 0.000** | 0.003** | ‐ |
H e and H o were calculated in arlequin. bottleneck analysis of each population and separate pooled populations within each of the three genetic lineages was performed using the two‐phase model with 95% single‐step mutations and a variance of 12 among multiple steps. *p < 0.05; **p < 0.01. N e: Current effective population size generated by bottleneck. Freq(private allele): frequency of private alleles.