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. 2019 Jun 27;8(13):e012609. doi: 10.1161/JAHA.119.012609

Figure 4.

Figure 4

Generation, phenotype, and gene expression of microRNA (miR)‐33 wild‐type (WT) and miR‐33 knock out knock in (KOKI) mice. A, Representative polymerase chain reaction (PCR) genotyping results and nomenclatures of the 4 strains of mice. B, Body and liver weight of 8‐week‐old miR‐33 4 strain mice. n=5 for knockout, n=5 for WT, n=6 for KOKI, and n=6 for KI for body weight. n=5 for knockout, and n=4 for WT, KOKI, and KI for liver weight. C, Serum lipid levels of the 4 strains of mice. n=13 for knockout, n=24 for WT, n=24 for KOKI, and n=14 for KI. D, Absolute copy number of miR‐33a and miR‐33b in miR‐33 WT mice and miR‐33 KOKI mice liver. n=4 for each. E, Quantitative PCR analysis for miR‐33 host and target genes in the liver. F, Densitometric analysis of Western blotting of miR‐33 target genes in the liver. G, Representative Western blotting images of miR‐33 target genes in the liver. H, Quantitative PCR analysis for low‐density lipoprotein‐cholesterol (LDLC) related genes in the liver. n=5 for WT, and n=6 for KOKI. Horizontal bars in the dot plots indicate mean±SEM. ABCA indicates ATP‐binding cassette A; HDLC, high‐density lipoprotein‐cholesterol; T‐Chol, total cholesterol; ACTB, Beta acitin; CPT, Carnitine Palmitoyltransferase; KO, Knock out; TG, Triglyceride. *P<0.05 compared with WT.