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. 2019 May 29;9(12):7157–7172. doi: 10.1002/ece3.5287

Table 1.

Morphological stages at which Baltic Mytilus edulis‐like larvae were sampled during the experiment

Stage Description Hours postfertilization
Control families Reared under low C T conditions
F001 F002 F003 F004
Early trochophore Precalcification 20 20 20 20 20
Trochophore Larvae exhibit dorsal flattening at the region of the shell field which marks the onset of calcification 22 22 22 22 22
Trochophore First trace of mineralization is observed by the presence of a small (ca. 20 µm) shell and birefringence at the hinge area. Presence of an early trochophore shell has previously been observed at 22 hpf (Ramesh et al., 2017) 24 25 25 24 25
Trochophore 49% ± 7.7% of the larval shell is covered by a mineralized shell 27 29 29 28 28
Late trochophore 76.9% ± 7.9% of the larval shell is covered by a mineralized shell 30 33 32 31 31
D‐veliger Larvae have secreted the PD I shell and exhibit a distinctive “D” shape 35 41 35 36 36

Changes in shell cover are quantified from N = 20 larvae and are reported as mean ± SD. Under control conditions, larval development across families was relatively uniform (maximum standard deviation = ca. +7% shell cover, Table 1) and sampled at six identical time points.