Table 1.
Stage | Description | Hours postfertilization | ||||
---|---|---|---|---|---|---|
Control families | Reared under low C T conditions | |||||
F001 | F002 | F003 | F004 | |||
Early trochophore | Precalcification | 20 | 20 | 20 | 20 | 20 |
Trochophore | Larvae exhibit dorsal flattening at the region of the shell field which marks the onset of calcification | 22 | 22 | 22 | 22 | 22 |
Trochophore | First trace of mineralization is observed by the presence of a small (ca. 20 µm) shell and birefringence at the hinge area. Presence of an early trochophore shell has previously been observed at 22 hpf (Ramesh et al., 2017) | 24 | 25 | 25 | 24 | 25 |
Trochophore | 49% ± 7.7% of the larval shell is covered by a mineralized shell | 27 | 29 | 29 | 28 | 28 |
Late trochophore | 76.9% ± 7.9% of the larval shell is covered by a mineralized shell | 30 | 33 | 32 | 31 | 31 |
D‐veliger | Larvae have secreted the PD I shell and exhibit a distinctive “D” shape | 35 | 41 | 35 | 36 | 36 |
Changes in shell cover are quantified from N = 20 larvae and are reported as mean ± SD. Under control conditions, larval development across families was relatively uniform (maximum standard deviation = ca. +7% shell cover, Table 1) and sampled at six identical time points.