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. 2019 May 22;10:636. doi: 10.3389/fpls.2019.00636

Figure 1.

Figure 1

Co-expression of PtdIns(3)P-binding proteins and StRem1.3 produces BiFC complexes distributed in large patches on the PM of N. benthamiana leaf cortical cells. (A) BiFC fluorescence in cells co-expressing VAM7-PX-VenusN or Hrs-2xFYVE-VenusN with VenusC-StRem1.3. The dotted boxes indicate the regions zoomed in in the top layer projections. (B) BiFC fluorescence in cells co-expressing VenusC-StRem1.3 with VenusN fusions to the PtdIns(3)P-non-binding mutants, VAM7-PX*, Hrs-2xFYVE*. Large membrane patches are highlighted by the solid circles, and small punctae are highlighted with the dotted circles.All scale bars represent 10 μm.