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. 2019 May 22;10:636. doi: 10.3389/fpls.2019.00636

Figure 2.

Figure 2

Membrane patches can be formed in N. benthamiana leaf cortical cells only by BiFC complexes containing a protein targeted to the PM and a protein targeted to multi-vesicular bodies (MVBs) and the tonoplast. (A) Peripheral membrane proteins BIK1, PBS1, CPK21, or PtdIns(4)P-binding protein FAPP1a fused to VenusN could produce membrane patches when co-expressed in BiFC complexes with VenusC-VAM7-PX or VenusC-Hrs-2xFYVE. (B) Fluorescence distribution of BiFC complexes containing only PM-targeted peripheral membrane proteins (PMPs). VenusN-fused BIK1, PBS1, CPK21, FAPP1a-PH were paired with VenusC-StRem1.3, as indicated. (C) Fluorescence distribution of BiFC complexes containing one PMP targeted to the PM and one PMP targeted to MVBs and the tonoplast. VenusN-fused RHA1, ARA7, ARA6, or RABG3f were paired with VenusC-StRem1.3 or FAPP1a-PH-VenusC, as indicated. (D) Fluorescence distribution of BiFC complexes containing only PtdIns(3)P biosensors and peripheral membrane proteins targeted to MVBs and the tonoplast. VenusC-Hrs-2xFYVE was paired with VenusN-fused ARA7, ARA6, VAM7-PX, or Hrs-2xFYVE. Scales are identical in all panels, representing 10 μm.