TABLE 1.
Gene | Primer orientation | Nucleotide sequence |
GAPDH | Forward (5′-3′) | 5′-TGACACTATGCAAGGTCGTTTCAC-3′ |
Reverse (5′-3′) | 5′-TCAGAACCGTCTAACTCTTGGTGG-3′ | |
mecA | Forward (5′-3′) | 5′-TACTGCTATCCACCCTCAAACA-3′ |
Reverse (5′-3′) | 5′-ATTTCACCTTGTCCGTAACCTG-3′ | |
femA | Forward (5′-3′) | 5′-AGCGTGTGTTAGTGCCTTTAGCGT-3′ |
Reverse (5′-3′) | 5′-CCATTGCACTGCATAACTTCCGGC-3′ | |
femB | Forward (5′-3′) | 5′-TTACAGAGTTAACTGTTACC-3′ |
Reverse (5′-3′) | 5′-ATACAAATCCAGCACGCTCT-3′ | |
femX | Forward (5′-3′) | 5′-TCGTGACGGTGAAGTTCAGG-3′ |
Reverse (5′-3′) | 5′-CACGCGTTAAGAAGCCATCG-3′ |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mecA, methicillin determinant A; femA, factor A essential for methicillin resistance; femB, factor B essential for methicillin resistance; femX, factor C essential for methicillin resistance.