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. 2019 Jul 22;10:1638. doi: 10.3389/fmicb.2019.01638

TABLE 1.

Primers for RT-qPCR.

Gene Primer orientation Nucleotide sequence
GAPDH Forward (5′-3′) 5′-TGACACTATGCAAGGTCGTTTCAC-3′
Reverse (5′-3′) 5′-TCAGAACCGTCTAACTCTTGGTGG-3′
mecA Forward (5′-3′) 5′-TACTGCTATCCACCCTCAAACA-3′
Reverse (5′-3′) 5′-ATTTCACCTTGTCCGTAACCTG-3′
femA Forward (5′-3′) 5′-AGCGTGTGTTAGTGCCTTTAGCGT-3′
Reverse (5′-3′) 5′-CCATTGCACTGCATAACTTCCGGC-3′
femB Forward (5′-3′) 5′-TTACAGAGTTAACTGTTACC-3′
Reverse (5′-3′) 5′-ATACAAATCCAGCACGCTCT-3′
femX Forward (5′-3′) 5′-TCGTGACGGTGAAGTTCAGG-3′
Reverse (5′-3′) 5′-CACGCGTTAAGAAGCCATCG-3′

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mecA, methicillin determinant A; femA, factor A essential for methicillin resistance; femB, factor B essential for methicillin resistance; femX, factor C essential for methicillin resistance.