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. 2019 Mar 5;17(8):1679–1693. doi: 10.1111/pbi.13093

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of SPL35 in Nicotiana benthamiana leaf epidermal cells. (a) SPL35 may be a peripherally membrane‐associated protein. Total extract from rice protoplasts was ultracentrifuged at 100 000 g for 1 h to obtain the pellets (P100) and supernatants (S100) fractions, followed by immunoblot analysis with anti‐GFP and antibodies as indicated. (b–f) Co‐expression of GFP‐SPL35 and OsAlaAT1‐mCherry (Cytosol marker), mCherry‐HDEL (ER marker), GmManI‐mCherry (Golgi marker), mCherry‐AtSYP61 (TGN marker) and mCherry‐AtVSR2 (PVC marker) in Nicotiana benthamiana epidermal cell‐derived protoplasts. Scale bars, 20 μm.