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. Author manuscript; available in PMC: 2020 Jun 5.
Published in final edited form as: J Am Chem Soc. 2019 May 13;141(22):8798–8806. doi: 10.1021/jacs.9b00045

Figure 2.

Figure 2.

FAHFA-TGs can be synthesized in cells. 13C4-PAHSA-containing triacylglycerols were synthesized after incubating differentiated 3T3-L1 adipocytes with 20 μM 13C4-PAHSA for 24 hours. The synthesis was inhibited by co-treating the cells with both DGAT1 inhibitor (1i) and DGAT2 inhibitor (2i). Shown is 13C4-PAHSA_16:1_18:1-TG level. N = 3, *p < 0.05, data are means ± SEM. The bottom panel shows the extracted ion chromatograms of two transitions for 13C4-PAHSA_16:1_18:1-TG. Red curve, transition corresponding to the loss of 13C4-PA plus ammonia; Blue curve, transition corresponding to the loss of 13C4-PAHSA plus ammonia.