Figure 3.
(A) CLSM images of CEM cells incubated with Dil-labeled sgc8-Exos (i) or lib-Exos (ii) at 37 °C for 2 h, and then stained with LysoTracker green and Hoechst 33258. The scale bar represents 30 μm. (B) Histogram analysis of the intracellular fluorescence signal of CEM cells treated with Dil-labeled sgc8-Exos or Exos at different conditions, in which 4 °C and NaN3 were used to inhibit energy-dependent endocytosis, and amiloride, chlorpromazine, M-β-CD, and dynasore were used to inhibit specific endocytosis pathways.