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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Nat Cell Biol. 2019 May 6;21(6):768–777. doi: 10.1038/s41556-019-0317-2

Figure 4. Mitochondria that colocalize with VGLUT1 are unmoved by Kif1a recruitment.

Figure 4

a, Axonal kymographs from live- imaging of neurons co-transfected with Kif1a(1-489)-DHFR-myc, Tom20-mCherry-FKBP and the presynaptic marker VGLUT1-Venus, before addition of 1μM zapalog (1) and after addition of zapalog (2). The mitochondria that are stationary in the presence of zapalog constitute the anchored pool.

b, Quantification of the number of stationary mitochondria before and after addition of zapalog, subdivided into two groups: stationary mitochondria that colocalize with VGLUT1-Venus and stationary mitochondria that do not colocalize with VGLUT1-Venus. (n=28 axons / 4 independent repeats; two-sided paired t-tests; ns = p>0.05, **** =p≤0.0001, source data in Sup. Table 1).