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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Nat Cell Biol. 2019 May 6;21(6):768–777. doi: 10.1038/s41556-019-0317-2

Figure 6. Mitochondria tend to be captured at axonal hotspots that are VGLUT1positive.

Figure 6

- a, Axonal kymographs from live- imaging of neurons co-transfected with Kif1a(1-489)-DHFR-myc, Tom20-mCherry-FKBP and the presynaptic marker VGLUT1-Venus. Axonal mitochondria were imaged: (1) prior to zapalog addition, (2) during zapalog-induced mitochondrial mobilization, and (3) following photolysis as they returned to the imaged region. Mitochondrial capture events were defined as any event wherein a motile mitochondrion becomes stationary for at least 5min.

b, Quantification of the number of capture events that coincided with a space that contained a stationary mitochondrion prior to zapalog addition vs. capture events at all other loci along the segment (normalized to μm of mitochondria-occupied axon and μm of remaining axon).

c, Quantification of the number of capture events that coincided with anchored mitochondria vs. capture events at all other loci along the segment (normalized to μm of anchored mitochondria and μm of remaining axon).

d, Quantification of number of capture events that coincided with presynaptic loci (defined as stationary VGLUT1-Venus in phase 1) vs. capture events at an equal number of randomly generated loci (see methods)

(n=11 axons / 3 independent repeats; box-and-whisker plots display statistical median (center value), upper and lower quartiles (boxes), max values (whiskers), and outliers (dots); two-sided paired t-tests; *** = p≤0.001, **** =p≤0.0001, source data in Sup. Table 1.