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. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Biochemistry. 2019 Feb 20;58(9):1260–1274. doi: 10.1021/acs.biochem.8b01053

Figure 5.

Figure 5.

Comparison of reactive amino groups and SuccK levels in αA and αB succinylated in vitro. The TNBS assay determined the reactive amino groups in αA and αB (A). A Western blot of unsuccinylated and succinylated αA and αB (with 0.01 mM SuccCoA) was performed using a SuccK antibody (B). Coomassie blue staining of the gel (shown are after protein transfer) showed equal loading of the proteins in the samples (C). A densitometric plot for the Western blot is shown in panel D. The bar graphs indicate the means ± SD of triplicate measurements. M, molecular weight markers. ***p < 0.0005.