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. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Biochemistry. 2019 Feb 20;58(9):1260–1274. doi: 10.1021/acs.biochem.8b01053

Figure 8.

Figure 8.

The effect of succinylation on the chaperone activity of αB. DTT-induced aggregation assay of insulin at 25 °C (A) and heat-induced aggregation assay of CS at 43 °C (C) in the absence and presence of αB and succinylated αB (generated at αB/SuccCoA molar ratios of 1:0.001, 1:0.003, 1:0.007, and 1:0.01). Total volume of assay mixture = 500 μL. Percent protection by αB and succinylated αB against insulin aggregation (B) and CS aggregation (D). DTT-induced aggregation assay of insulin in the absence or presence of αB and Succ-αB precomplexed with CS (E). Percent protection by αB and Succ-αB (generated at αB/SuccCoA molar ratios of 1:0.001) precomplexed with CS against insulin aggregation (F). The bar graphs indicate the means ± SD of triplicate measurements. *p < 0.05, **p < 0.005, ***p < 0.0005.