Skip to main content
. Author manuscript; available in PMC: 2019 Jul 29.
Published in final edited form as: Cell Rep. 2019 Jul 16;28(3):792–803.e4. doi: 10.1016/j.celrep.2019.06.044

Figure 3. MCs Inhibited SAT PDGFRα+ Beige Progenitor Cell Proliferation and Browning.

Figure 3

(A) Real-time PCR analysis of beige cell markers, beige progenitor marker Pdgfrα, and pre-adipocyte markers in SAT from the indicated groups of mice (n = 8–13 per group).

(B) FACS analysis of PDGFRα+ cells in SAT (as defined by CD34+ PDGFRα+). n = 8~10 per group.

(C) Immunofluorescent double staining of PDGFRα+ (red) and UCP1+ (green) double-positive cells.

(D) Immunofluorescent double staining of PDGFRα+ (red) and Ki67+ (green) double-positive cells in SAT, with nuclei counterstained with DAPI (blue) (n = 8 per group).

Scale bars, 100 µm; inset scale bars, 25 µm. Representative images in (B)–(D) are shown on the left.

Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.