| Antibodies |
|
| Anti-mouse PDGFRα (CD140a)-APC |
BioLegend |
Cat# 135907; RRID:AB_2043969 |
| Anti-CD34-FITC |
eBioscience |
Cat# 11–0341-85; RRID:AB_465022 |
| Anti-CD45-PE |
eBioscience |
Cat# 12–0451-83; RRID:AB_465669 |
| Anti-CD117-APC |
eBioscience |
Cat# 17–1171-82; RRID:AB_469430 |
| Anti-FCεR1-PE-CY7 |
eBioscience |
Cat# 25–5898-82; RRID:AB_2573493 |
| Anti-mouse PDGFRα |
R&D |
Cat# AF1062; RRID:AB_2236897 |
| Anti-mouse CD117 |
ebioscience |
Cat# 14–1171-85; RRID:AB_467434 |
| Anti-mouse UCP1 |
Abcam |
Cat# 10983; RRID:AB_2241462 |
| Anti-TPH1 |
Millipore |
Cat# AB15570; RRID:AB_877305 |
| Anti-Ki67 |
Novus |
Cat# NB600–1209; RRID:AB_10001641 |
| Anti-β-actin |
Sigma-Aldrich |
Cat# A1978; RRID:AB_476692 |
| Anti-GAPDH |
Cell Signaling Technology |
Cat# 2118; RRID:AB_561053 |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
| High-fat diet |
Research Diets |
Cat# D12492 |
| Control chow diet |
Research Diets |
Cat# D12450B |
| Norepinephrine |
Sigma-Aldrich |
Cat# A7257 |
| Oil-red O |
Sigma |
Cat# O0625 |
| LDL-c |
Lee Biosolutions |
Cat# 360–10 |
| Type II collagenase |
Worthington |
Cat# LS004177
|
| RPMI 1640 |
GIBCO |
Cat# 61870–036 |
| DMEM |
GIBCO |
Cat# 10566–016 |
| LX1031 |
Medchem Express |
Cat# HY-13041 |
| Recombinant IL-3 |
PeproTech |
Cat# 213–13 |
| Recombinant stem cell factor |
PeproTech |
Cat# 250–03 |
| 3,3′,5-triiodo-L-thyronine (T3) |
Sigma-Aldrich |
Cat# T2877 |
| Isobutylmethylxanthine |
Sigma-Aldrich |
Cat# I7018 |
| Dexamethasone |
Sigma-Aldrich |
Cat# D4902 |
| Indomethacin |
Sigma-Aldrich |
Cat# I7378 |
| Rosiglitazone |
Sigma-Aldrich |
Cat# R2408 |
| Insulin |
Sigma-Aldrich |
Cat# 91077C |
| Haematoxylin |
Sigma-Aldrich |
Cat# GHS316 |
| Eosin |
Sigma-Aldrich |
Cat# HT110116 |
| Toluidine blue |
Sigma-Aldrich |
Cat# 89640 |
| DAPI |
Sangon Biotech |
Cat# E607303 |
| SYBR green dye |
TAKARA |
Cat# DRR081A |
|
| Critical Commercial Assays |
|
| Serotonin |
LDN |
Cat# BA E-8900 |
|
| Experimental Models: Cell Lines |
|
| 3T3-L1 |
ATCC |
Cat# CL-173 |
|
| Experimental Models: Organisms/Strains |
|
| Wild-type (C57BL/6J) |
Vital River Laboratory Animal Technology Co. Ltd. (Beijing, China) |
Cat# 213 |
|
Kitw-sh/w-sh (C57BL/6J) B6.Cg-KitW-sh/HNihrJaeBsmJ |
Jackson Laboratory (purchased during last 7 years and bred at Hefei) |
Cat# 030764 |
|
Tph1–/– mice (C57BL/6J) |
University of Wisconsin-Madison |
N/A |
|
| Software and Algorithms |
|
|
|
| Image-Pro plus 6.0 |
Media Cybernetics, Inc. |
http://www.mediacy.com/imageproplus |
| Kaluza Analysis |
Beckman Coulter |
https://en.freedownloadmanager.org/Windows-PC/Kaluza-Analysis.html |
