Figure 2.
In vitro RNAi screening experiments suggested six genes - CD27, CEACAM1, CTLA4, LRIG1, PDCD1LG2, and TNFRSF18 as tumor suppressors. Frequency histogram analysis showed that the shRNAs (short hairpin RNAs) targeting one of the six genes were ranked within the top 1% highly expressed shRNAs from the genome-scale library of ∼100,000 pooled shRNAs targeting about 17,080 genes. Plots of the first 22 cell lines for the genes - CD27, CEACAM1, CTLA4, and LRIG1 were shown.