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. 2019 Jul 29;9:10898. doi: 10.1038/s41598-019-45319-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibodies-added (Combined) IF expression of pho-4EBP1 and pho-S6K1 for both the control group and the 500 nM everolimus group after 24 hours. (a) Everolimus-sensitive cells (Hs578T, MCF7 cell lines). (b) Everolimus-resistant cells (MDA-MB-231, BT474 cell lines and ABC-16TX1 PDCC).