|
Target
|
Protein expressions |
Protein expressions |
Protein expressions |
|
Sample acquisition
|
Proteins lysated form cells or tissues |
Tissues |
Cells or tissues (supension or adherent cells) |
|
Sample quantity
|
cells typically (about 15–30 μg total proteins) |
cells from tissues typically |
cells typically (1/10–1/100 times compared with amount of western blot) |
|
Advantages
|
Sensitivite detection |
Can measure situations in the context of intact tissues. |
Can be used in suspension and adherent cells situations |
| Quantitative analysis use |
|
Can be used in fresh samples situations |
| Provides moleualr weight information |
|
Less sample need amount and less time to perform results |
|
|
Quantitative analysis |
|
|
Suitable for detection for phosho-proteins |
|
Disadvantages
|
Time consuming problem |
Less sensitivity quantitatively |
The condition setting for high sensitivity |
| High sample comsumption |
Limited time of storage |
Limited time of storage |
| Lower sensitivity in less protein amount situation |
Repeated use problem |
Repeated use problem |
| Improper quantification |
|
|
| Measuring confusion when phosphorlyative peotein detection |
|
|
| Reactivity problem of antibodies each other |
|
|
