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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Sep;38(9):2126–2136. doi: 10.1161/ATVBAHA.118.311402

Figure 4. Shear-induced NF-kB Activation and ICAM-1 expression are reduced in CSE−/− endothelium.

Figure 4.

(A) Intimal mRNA was collected 48 hours after partial ligation and assessed for VCAM-1 and ICAM-1 expression. n=4–8. (B) NF-κB phosphorylation following short-term oscillatory shear stress (OSS, 0 to 120 min) was prevented in CSE−/− MAECs. n=4. (C/D) NF-κB activation following chronic OSS (16 hours) was assessed by (C) phosphorylation and (D) nuclear translocation in WT and CSE−/− MAECs. For nuclear translocation, at least 80 cells were scored for nuclear NF-κB staining per condition within each experiment. n=4. (E/F) Chronic OSS-induced (E) ICAM-1 and (F) VCAM-1 expression was assessed by Western blotting. Representative images are shown. n=4. * p<0.05, ** p < 0.01, *** p<0.001 by Students T-test (A) or Two-Way ANOVA (B-F).