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. Author manuscript; available in PMC: 2019 Aug 4.
Published in final edited form as: Nature. 2019 Feb 4;566(7743):218–223. doi: 10.1038/s41586-019-0908-x

Figure 4. Distinct CasX conformational states.

Figure 4.

a, Conformational states with 30bp target DNA; b, with a DNA target forming the full R-loop; and c, with the short NTS (20nt) and the 45nt TS. The schematic of the DNA probe used for each data collection is shown on the left, with cleavage sites shown by arrowheads. The top views of the cryo-EM maps for the CasX ternary complex in States I and II are shown on the center panels. The TS density is colored purple, the NTS is colored magenta, and the sgRNA density is colored teal. The RuvC domain is indicated in each map. All the EM maps are low-pass filtered to 4.5 Å. The relative percentage of particles belonging to each state revealed by cryo-EM analysis is shown in the right panel.