Figure 3.

Evaluation of senescence. To determine if the combination of HA (high molecular weight) and imatinib prevents the induction of senescence, both cell lines were treated with either each compound alone or with a combination of both for 48 h. SA-β-gal and SAHF were then evaluated. (A) SA-β-gal activity (upper panel). Results are expressed as means ± SEM of three independent experiments ^##p < 0.01 treated vs. untreated cells, while **p < 0.01 and *p < 0.05 between the treatments indicated (lower panel). Pictures are from a representative experiment (B) SAHF, magnification 400X. Arrows indicate nuclei with SAHF, while lines indicate nuclei with DNA fragmentation, which are characteristic of senescent and apoptotic cells, respectively.