. 2019 Jul 29;9:10988. doi: 10.1038/s41598-019-47230-4

Table 1.

Thermodynamic parameters of M-TTR and W79F M-TTR urea-induced unfolding measured with conventional fluorescence and second derivative spectra^a.

Parameter	Gross fluorescence at 362 nm	d²F/dx² at 325 nm	d²F/dx² at 348 nm	d²F/dx² at 364 nm	Gross fluorescence (ref.²⁶)
M-TTR
$Δ G_{U - F}^{H 20}$ (kJ mol⁻¹)	20.7 ± 3.5	21.3 ± 4.0	20.0 ± 8.0	19.7 ± 4.5	20.4 ± 1.5
m (kJ mol⁻¹ M⁻¹)	7.8 ± 1.3	7.1 ± 1.3	7.8 ± 2.9	7.7 ± 1.6	6.9 ± 0.5
C_m (M)	2.8 ± 0.2	3.0 ± 0.3	2.6 ± 0.3	2.5 ± 0.4	3.0 ± 0.1
W79F M-TTR
Parameter	Gross fluorescence at 330 nm	d ² F/dx ² at 324 nm	d ² F/dx ² at 346 nm	d ² F/dx ² at 367 nm	Gross fluorescence (ref.²⁶)
$Δ G_{U - F}^{H 20}$ (kJ mol⁻¹)	12.9 ± 1.3	12.9 ± 2.8	n.d	21 ± 14	13.0 ± 1.4
m (kJ mol⁻¹ M⁻¹)	4.2 ± 0.4	4.4 ± 0.08	n.d	6.6 ± 4.3	4.3 ± 0.4
C_m (M)	3.1 ± 0.2	3.0 ± 0.1	n.d	3.2 ± 0.2	3.0 ± 0.1

All data are derived from the data shown in Figs 5 and 6, except those of the last column, previously determined²⁶.