Table 1.

Kinetic parameters for proteolysis of V1 and V1 5-GAG by ADAMTS -1, -4 and -5 and their deletion variants.

Variant	Substrate	kcat/Kma 105 M−1 s−1	Fold reduction
ADAMTS-4
WT	V1	1.86 ± 0.37	—
WT	V1-5GAG	2.10 ± 0.28	—
MDTC	V1-5GAG	0.012 ± 0.005**	167
ADAMTS-5
WT	V1	34.6 ± 5.19	—
WT	V1-5GAG	35.6 ± 3.80	—
MDTCS	V1-5GAG	29.1 ± 3.36	1.2
MDTCS + 2B9	V1-5GAG	7.58 ± 1.50*	4.7
MDTC	V1-5GAG	1.86 ± 0.32*	19
MDT	V1-5GAG	0.23 ± 0.06*,†	153
MD	V1-5GAG	0.13 ± 0.03*	276

^aValues determined by time course experiments at 50 nM substrate concentration.

Results given in nanomolar and expressed as mean ± SEM. *p < 0.05, **p < 0.01, compared to wild-type (WT) enzyme. ^†p < 0.05 compared with MDTC (n = 3–9).