Fig. 3.
MPO-ANCA production in S. aureus pSJH101 6PGD391–410 immunized mice. a Serum IgG from S. aureus pSJH101-derived 6PGD391–410 immunized C57BL/6 mice (pooled, n = 8) binds to neutrophils from C57BL/6 mice, but not those from Mpo−/− mice in a perinuclear (pANCA) fashion. b Anti-MPO ELISA on sera from mice immunized with OVA323–339 (n = 8), MPO409–428 (n = 10), or pSJH101 6PGD391–410 (n = 10, values representative of two independent experiments. c Anti-MPO447–459 ELISA using pooled serum IgG from pSJH101 6PGD391–410-immunized mice, triplicates representative of two independent experiments. d S. aureus pSJH101 6PGD391–410 inhibits autoantibody binding to MPO409–428. Serum IgG from S. aureus pSJH101 6PGD391–410 immunized mice was pre-incubated with S. aureus pSJH101 6PGD391–410 then used to detect anti-MPO409–428 IgG antibodies by ELISA. Values are quadruplicates. e Neutrophil reactive oxygen species (ROS) production via rhodamine 123 (R123) induced by pooled serum IgG from pSJH101 6PGD391–410 immunized mice. Flow cytometric plots illustrate the data, performed in triplicate. f Glomerular neutrophil recruitment after injection of serum IgG from pSJH101 6PGD391–410 immunized mice (n = 5 each group). Photomicrographs illustrate the data, presented numerically as neutrophils per glomerular cross section (Cells/gcs). Scale bar is 30 μm. Error bars represent mean ± s.e.m. **P < 0.01, ***P < 0.001 by Kruskal–Wallis test (b, c), Mann–Whitney U-test (d, e, f). Source data are provided as a Source Data file