Fig. 5.
Anti-MPO T-cell responses after immunization S. aureus-derived 6PGD391–410 sequences. C57BL/6 mice (n = 4 each group) were immunized with either a MPO409–428 (positive control), b pSJH101-derived 6PGD391–410 (YFKNIVTDYQEALRDVVATG), c S. aureus 6PGD391–410 Variant 1 (YFKNIVTDYQDALRDVVATG), d S. aureus 6PGD391–410 Variant 2 (YFKNIVTNYQEALRDVVATG), e S. aureus 6PGD391–410 Variant 3 (YFKNIVTEYQDALRDVVATG), f S. aureus 6PGD391–410 Variant 4 (YFKNIVTNYQDALRDVVATG). T-cell recall responses were measured ex vivo to either OVA 323–339, the immunizing peptide, or MPO409–428 using [3H]-thymidine proliferation assays (top row), and ELISPOT IFN-γ (middle row) or IL-17A (bottom row). Each dot represents the response from an individual mouse, error bars represent the mean ± s.e.m. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by Kruskal–Wallis test. Source data are provided as a Source Data file