Fig. 6.
MPO-ANCA after immunization with S. aureus-derived 6PGD391–410 sequences. a Perinuclear (pANCA) detection using purified serum IgG, pooled from each group of C57BL/6 mice (n = 8 each group) immunized with OVA323–339, MPO409–428, pSJH101-derived 6PGD391–410 (YFKNIVTDYQEALRDVVATG), S. aureus 6PGD391–410 Variant 1 (YFKNIVTDYQDALRDVVATG), S. aureus 6PGD391–410 Variant 2 (YFKNIVTNYQEALRDVVATG), S. aureus 6PGD391–410 Variant 3 (YFKNIVTEYQDALRDVVATG), S. aureus 6PGD391–410 Variant 4 (YFKNIVTNYQDALRDVVATG) (amino acid substitutions underlined). Nuclei are blue (DAPI), mouse IgG is green (FITC-conjugated anti-mouse IgG antibody). b Sera from individual mice (n = 8 each group) immunized with pSJH101-derived 6PGD391–410 and other S. aureus-derived sequences of 6PGD391–410 tested by an anti-rmMPO ELISA. c Anti- MPO447–459 antibody detection by ELISA using purified serum IgG pooled from each group of C57BL/6 mice (a). Scale bar is 5 μm (all photomicrographs). Error bars represent the mean ± s.e.m. of triplicates. **P < 0.01, ***P < 0.001 by Kruskal–Wallis test. Source data are provided as a Source Data file