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. 2019 Jul 29;10:3362. doi: 10.1038/s41467-019-11325-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Reduced phosphorylation of MLC2 and ERM in Par3eKO mice. a Immunohistochemistry of P100 murine back-skin paraffin cross-sections stained for pMLC2 (Ser19) (gray). Left panels: overview micrographs (×20 objective, scale bar: 100 µm). Middle and right panel: higher magnification micrographs referring to signals within the IFE (middle panel) and HF (right panel). ×40 objective, scale bar: 40 µm (IFE), 20 µm (HF). b Quantification of pMLC immunoreactivity a. Intensity in arbitrary units. n = 4 mice, Mann–Whitney U-test, *p = 0.0286, mean ± SD. c Immunohistochemistry of P100 murine back-skin paraffin cross-sections stained for ppMLC2 (Thr18/Ser19) (gray) (Cell Signaling Technologies #3674). ×63 objective, scale bar: 20 µm. d Quantification of ppMLC immunoreactivity c. Intensity in arbitrary units, n = 6 mice, Mann–Whitney U-test, *p = 0.0411, mean ± SD. e Immunohistochemistry of P100 murine back-skin paraffin cross-sections stained for pERM (gray). Left panels: overview micrographs (×20 objective, scale bar: 100 µm). Middle and right panel: higher magnification micrographs referring to signals within the IFE (middle panel) and HF (right panel). ×63 objective, scale bar: 20 µm (IFE and HF). Image intensity was enhanced for better visualization (×63). f Quantification of pERM immunoreactivity e. Intensity in arbitrary units. n = 4 mice, Mann–Whitney U-test, mean ± SD. In all micrographs DAPI is shown in blue. Ctrl control, IFE interfollicular epidermis, HF hair follicle, ns non-significant