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. 2019 Jul 29;9:10984. doi: 10.1038/s41598-019-47387-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FGF2 promotes the expression of E-cadherin, laminin-binding integrins, & Aqp5. Representative confocal images of mSG-PAC1 spheroids cultured in EGF or FGF2-containing medium for five days in a Matrigel/collagen I matrix and stained for (a) E-cadherin, integrin α3 subunit, or integrin α6 subunit and DNA, or (c) Aqp5. Images are maximum projection images of five z-slices acquired at 40X taken in 0.4 μm steps. (b & d) Fluorescence intensity was normalized to the number of nuclei per field from 13 spheres from three independent experiments and plotted relative to the expression in EGF ± s.e.m. Size bar, 50 μm. Data was analyzed by Student’s T-test. P < 0.05–0.0001, as indicated.