Table 1.
Antibodies.
| Antibody | Company | Catalog # | Dilution | Application |
|---|---|---|---|---|
| Primary Antibodies | ||||
| AQP5 | Alomone Labs | AQP-005 | 1:200 | ICC |
| Laminin | Abcam | Ab11575 | 1:400 | ICC |
| Laminin α5 | Gift from Lydia Sorokin | 1:400 | ICC | |
| Cytokeratin 7 | Abcam | Ab9021 | 1:100 | ICC |
| Cytokeratin 19, TROMA-III | DSHB | AB-2133570 | 1:100 | ICC |
| E-cadherin | BD Biosciences | 610182 | 1:200 | ICC |
| Integrin α6 | BD Biosciences | 555734 | 1:200 | ICC |
| Integrin α3 | C.M. DiPersio | 1:200, 1:1000 | ICC, WB | |
| Sox10 | Santa Cruz | sc-17342 | 1:200 | ICC |
| Cdc42 | Santa Cruz | sc-8401 | 1:500 | WB |
| RhoA | Santa Cruz | sc-179 | 1:500 | WB |
| GAPDH | Invitrogen | MA5-15738 | 1:3000 | WB |
| Secondary Antibodies | ||||
| AF568 Donkey anti-Rabbit | Alexa-Fluor | A10042 |
1:1000 (2D) 1:500 (3D) |
ICC |
| AF488 Donkey anti-Mouse | Alexa-Fluor | A21202 |
1:1000 (2D) 1:500 (3D) |
ICC |
| AF488 Goat anti-Rat | Alexa-Fluor | A11006 |
1:1000 (2D) 1:500 (3D) |
ICC |
| AF488 Donkey anti-Goat | Alexa-Fluor | A11055 |
1:1000 (2D) 1:500 (3D) |
ICC |
| AF568 Goat anti-Rat | Alexa-Fluor | A11077 |
1:1000 (2D) 1:500 (3D) |
ICC |
| AF568 Donkey anti-Goat | Alex-Fluor | A11057 |
1:1000 (2D) 1:500 (3D) |
ICC |
| DRAQ5 | Cell Signaling Technology | 4084 |
1:1000 (2D) 1:500 (3D) |
ICC |
Immunocytochemistry (ICC).
Western Blotting (WB).