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Table 2.
p53/α3fl/fl PCR Protocol.
| Temperature (°C) | Time | # Cycles |
|---|---|---|
| 94 | 2 min | 1 |
| 94 | 30 sec | 33 |
| 55 | 30 sec | |
| 72 | 45 sec | |
| 72 | 5 min | 1 |