Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 29;9:10919. doi: 10.1038/s41598-019-47227-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Nuclear alpha-synuclein is rapidly recruited to sites of laser-induced DNA damage in vivo. (A) Cortical neuron cell body imaged in vivo in a mouse expressing 142E Syn-GFP (heterozygous) and nuclear localized TdTomato-NLS (heterozygous). White circle in merge image shows targeting of laser-induced damage (LID) pulse and white square is the control region. Dotted line represents the outline of the nucleus. Scale bar 5 μm. (B) Baseline (t = −1min) and after LID (t = 1 min) images show accumulation of Syn-GFP at DNA damage site (white arrow). (C) Data from B) shows increased (at LID site) and decreased (at adjacent site, square in A) Syn-GFP level, calculated enrichment ratio at LID site, and group data from different transgenic (142E Syn-GFP & A53T Syn-GFP) and AAV8-mediated expression (A53T/S129D Syn-GFP & A53T/S129A Syn-GFP) animals (142E Syn-GFP Enrichment Ratio = 1.26 ± 0.03, N = 34 cells/6 animals, A53T Syn-GFP Enrichment Ratio = 0.95 ± 0.02, N = 19 cells/3 animals, A53T/S129D Syn-GFP Enrichment Ratio = 1.37 ± 0.13, N = 17 cells/5 animals, A53T/S129A Syn-GFP Enrichment Ratio = 1.00 ± 0.04, N = 13 cells/4 animals; F(3, 78) = 9.086, p = 0.0001, ANOVA, post-hoc Tukey tests: A53T/S129D vs. A53T p = 0.0002, A53T/S129D vs. A53T/S129A p = 0.0051, 142E vs. A53T p = 0.0016, 142E vs. A53T/S129A p = 0.0343, A53T/S129D vs. 142E p = 0.6078, A53T vs. A53T/S129A p = 0.9726). (D) White square shows area magnified to the right. FRAP shows rapid mobility of Syn-GFP within LID site (white arrow, τ_recovery = 33.1 ms, 95% CI = 23.8–54.4 ms, N = 12 cells/3 animals). Red arrowheads in all sections show time of LID or FRAP laser pulse.