Figure 5.

Conditional knockdown of NCAPG inhibits tumor growth in vivo. A) Western blot analysis showing the specific dox-mediated knockdown of NCAPG in cells with tetracycline-inducible shRNA against NCAPG. B) Colony formation assay showing dox-induced NCAPG knockdown cells formed significantly fewer colonies. C) Cell cycle analysis using propidium iodide staining and FACS showing a significantly reduced cell population in S and G₂/M phase and a correspondingly higher cell population in G₁ and sub-G₁ phase in dox-induced NCAPG knockdown cells compared with control cells. D) Representative mouse xenograft imaging showing dox-induced NCAPG knockdown cells formed significantly smaller tumors. E) Tumor volume of mouse xenograft tumor formed by cells containing tetracycline-inducible shRNA against NCAPG or control with or without dox treatment, over time. F) Boxplot of final tumor weight of xenograft tumors shown in E. *P < 0.05.