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. 2019 Jun 4;33(8):9540–9550. doi: 10.1096/fj.201802298R

Figure 5.

Figure 5

Rotenone treatment causes muscle damage but not calcium overload in C. elegans. A) Adult synchronized animals were treated with 0 (C, control), 2, 4 and 10 µM Rotenone for 36 h with or without MMP inhibitor (10 µM) or Furin inhibitor (1 µM), after which they were scored for muscle damage. Significant muscle damage was found in 10 µM rotenone, which could not be rescued with MMP or Furin inhibitor. Number of muscle images/conditions (269, 231, 144, 197, 280, 228, 207, 112, 257, 155, 217, and 169 from left to right). B) Adults expressing GCaMP3.35 were treated with 0 (C, control), 2, 4 and 10 µM Rotenone for 36 h, after which they were imaged using fluorescence microscopy with objective lens, ×10 magnification, and the calcium level quantified using ImageJ software. No significant change in calcium was observed in all 3 concentrations. Five animals were analyzed for each condition. Inh., inhibitor. Letters (a, b) on top of bars indicate statistical significance; X, median value.