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. 2019 May 7;33(8):9182–9193. doi: 10.1096/fj.201900321R

Figure 3.

Figure 3

sKlotho inhibits TRPC6 currents independent of FGFR and FGF23. A, B) sKlotho inhibits DAG-activated TRPC6 expressed in HEK cells (A) and in L6 cell line lacking endogenous FGFRs (B). Inset is I-V relationship curves for DAG-stimulated currents. OAG (a membrane-permeant analog of DAG, 100 μM) was used to activate TRPC6. Bar graph shows means ± sem of maximal DAG-stimulated inward TRPC6 current density (∆ current ± DAG in picoamperes at −100 mV divided by cell capacitance in picofarads). C) FGF23 has no effect on TRPC6, nor does it affect sKlotho regulation of TRPC6. FGF23 (400 pM) and sKlotho (100 pM) were used. sKL, sKlotho; n = 7–10 each group. *P < 0.05 vs. control (first bar from left, no sKL).