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. 2019 May 7;33(8):9182–9193. doi: 10.1096/fj.201900321R

Figure 7.

Figure 7

Evidence for CBM regulating TRPC6 via binding sialic acid, involving lipid rafts. A) Preincubation with α2-3-sialyllactose (3′-SiaLac), but not with lactose (Lac), prevents inhibition of TRPC6 by CBM. 3′-SiaLac, Lac, and CBM were premixed with 100 μl of conditioned media and incubated for 1 h, then added into cells and incubated for 3 h. n = 6–8 each group. *P < 0.05 between indicated groups. Veh, vehicle; N.S., statistically not significant. B) No additivity of inhibitory effects among CBM, PI3K inhibitor WMN, and filipin (cholesterol sequester–lipid raft inhibitor). Where indicated, cells were treated with sKL, CBM, WMN, filipin, or a combination for ∼3 h prior to recording the current. *P < 0.01 vs. control (first bar from left); n = 9–12 each group.