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NHERF1 knockdown does not affect cAMP accumulation in human ASM cells. A) NHERF1 knockdown was confirmed by immunoblotting; a representative blot is shown. B) After SCR or NHERF1 siRNA transfection, cells were stimulated with cAMP-elevating agents for 10 min in the presence of IBMX. Data are means ± sem; n = 3.
