Figure 5.

NHERF1 knockdown does not limit cAMP-driven CREB signaling in human ASM cells. A) Cells were stimulated with ISO (1 µM; 30–240 min), and p-CREB was determined in whole cell lysates. B) Human telomerase reverse transcriptase–expressing human ASM cells stably transfected with CRE-Luc reporter construct were stimulated with ISO (1 µM), PGE₂ (1 µM), or FSK (10 µM) for 6 h, lysates were collected, and luciferase activity was determined. C) For cyclin D1 expression analysis, cells were stimulated with PDGF (10 ng/ml) with or without ISO (1 µM), PGE₂ (1 µM), or FSK (10 µM) post–siRNA transfection and cell lysates collected. Representative blots are shown. Data are means ± sem; n = 3. Two-way ANOVA followed by Bonferroni multiple comparison test. *P < 0.05 vs. own SCR control.