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. 2019 May 24;33(8):9516–9525. doi: 10.1096/fj.201900428RR

Figure 3.

Figure 3

ER stress increases production of ROS. Inhibition of ROS production by NAC partially reduces formation of lipid droplets but does not prevent synthesis of NP. ROS were measured by fluorescein emission as described in Materials and Methods. A) Both salubrinal (Sal; 100 μM) and the reductant NAC (5 mM) individually reduce ROS both in uninfected cells (MOCK) and infected (I) cells. B, C) NAC partially (25%) suppresses the production of lipid droplets stimulated by infection (P < 0.001 for NAC vs. I+NAC and P < 0.05 for IAV+NAC vs. NAC). D) However, NAC does not suppress production of viral NP. At least 3 separate experiments were run for each condition. Measurements were at 24 HPI. In B and D, original magnifcation is ×400 and full field is shown. SEM by Student’s t-test are shown. The values indicated are arbitrary fluorescence values. *P < 0.05, ***P < 0.001.