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. 2019 Jul 29;14(7):e0220184. doi: 10.1371/journal.pone.0220184

Fig 3. Pv33 induces cell death in V. vinifera cv Syrah.

Fig 3

Agrobacterium-mediated transient expression of Pv18, Pv31, Pv33, Pv82 without their signal peptide (Δsp) and full-length Pv33, including its signal peptide (FL) in V. vinifera Syrah leaf disks. (A) Macroscopic results. Pictures were taken 6 days after agroinfiltration and are representative of the phenotype of each effector. (B) Semi-quantitative RT-PCR showing VvHSR and effector expression. Actin expression is used as reference. Agrobacterium-mediated transient expression of GFP was used to confirm that the induction of VvHSR expression is specific to Pv33. Each effector-expressing Agrobacterium strain was infiltrated into 4 leaf discs and the experiment was repeated twice. The macroscopic effect of 33Δsp was not visible in all infiltrated discs but could be observed in both repetitions. RNA extractions for RT-PCRs were done by pooling the 4 disks for each effector.