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Semi-quantitative RT-PCR of Pv33 expression in sporangia (Sp), germinated spores (Sg) and infected tissues at 0, 24, 48 and 72 hours after inoculation (hpi). Expression of V. vinifera Actin (VvActin) is shown as loading of samples corresponding to infected tissues. Expression of P. viticola Actin (PvActin) reflects pathogen biomass and shows progression of infection.
