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. 2019 Jul 29;14(7):e0220184. doi: 10.1371/journal.pone.0220184

Fig 7. Pv33 localizes to the nucleus.

Fig 7

(A) Confocal microscopy images following infiltration of N. benthamiana leaves with Agrobacterium carrying GFP translational fusions of Pv33 either lacking (33Δsp-GFP) or including (33FL-GFP) the signal peptide. GFP alone was used as control. (B) Low magnification epifluorescence microscopy images of 33ΔSP-GFP and 33FL-GFP infiltrations. (C) Epifluorescence microscopy images following infiltration of V. vinifera cv Syrah leaf discs with Agrobacterium carrying 33ΔSP-GFP and 33FL-GFP. Pictures were taken 2 days after agroinfiltration. Experiments were repeated 5 times for N. benthamiana and twice for V. vinifera, with the same results. Bar shows 25 µm.