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. 2019 Jul 29;14(7):e0220184. doi: 10.1371/journal.pone.0220184

Fig 8. Pv33-triggered cell death depends on its nuclear localisation.

Fig 8

(A) Agrobacterium-mediated transient expression of N. benthamiana leaves with 33Δsp-GFP fused to either an active (33Δsp-GFP-NES) or inactive (33Δsp-GFP-nes) nuclear exclusion sequence. Pictures were taken 5 days after agroinfiltration under white and blue light. (B) Subcellular localisation of 33Δsp-GFP-NES and 33Δsp-GFP-nes as observed by confocal microscopy 2 days after agroinfiltration. (C) Western blot with anti-GFP of the different GFP-tagged Pv33 versions. GFP is used as positive control. Ponceau red staining of the nitrocellulose membrane is shown as loading marker. Results shown in A and B are representative of 3 independent experiments, each consisting of at least 6 N. benthamiana leaves infiltrated as shown in A.