Table 1. Primer sequences for the real-time quantitative polymerase chain reaction .
RORɣt |
F: 5′AGGAAGTCCATGTGGGAG3′ R: 5′AGCACAATCTGGTCATTCTGG3′ |
IL-17 |
F: 5′CTCATTGGTGTCACTGCT3′ R: 5′CGGTTGTAGTAATCTGAGG3′ |
IL-22 |
F: 5′CAACAGGCTAAGCACATGTCATATTG 3′ R: 5′CAGGCATTTCTCAGAGACATAAACAG3′ |
β-actin |
F: 5′AGACGCAGGATGGCATGGG3′ R: 5′GAGACCTTCAACACCCCAGCC3′ |
F, forward primer; R, reverse primer; RORɣt, retinoic acid receptor-related orphan receptors gamma; IL-17A, interleukin-17A; IL-22, interleukin-22